In vivo CFTR assay by nasal potential difference (NPD) measurement

LR Lawrence W. Rasmussen
DS Denise Stanford
KP Krina Patel
SR S. Vamsee Raju
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In vivo CFTR activity was assessed in murine nasal epithelium by NPD measurements [29]. Nasal catheter made of PE10 tubing was inserted into a single naris of anesthetized mice for sequential infusion of Ringers solution (baseline); Ringers + Amiloride (100 μM); Chloride-free Ringers solution consisting of KHPO (2.4 mM), KHPO (0.4 mM), Na Gluconate (115 mM), NaHCO (25 mM), and Ca Gluconate (1.24 mM); Chloride-free Ringers + forskolin (20 μM); and Chloride-free Ringers with 10 μM of CFTR-inh172. CFTR function was expressed in mean changes in voltage.

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