In vivo CFTR activity was assessed in murine nasal epithelium by NPD measurements [29]. Nasal catheter made of PE10 tubing was inserted into a single naris of anesthetized mice for sequential infusion of Ringers solution (baseline); Ringers + Amiloride (100 μM); Chloride-free Ringers solution consisting of KHPO (2.4 mM), KHPO (0.4 mM), Na Gluconate (115 mM), NaHCO (25 mM), and Ca Gluconate (1.24 mM); Chloride-free Ringers + forskolin (20 μM); and Chloride-free Ringers with 10 μM of CFTR-inh172. CFTR function was expressed in mean changes in voltage.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.