The full-length ANK protein sequences from Arabidopsis and Capsicum species were aligned by using ClustalW as described82. The alignment file was then used to construct a phylogenetic tree based on the maximum-likelihood method of the MEGAX (Molecular Evolutionary Genetics Analysis) software83. The phylogenetic tree of the ANK proteins was displayed by using the interactive Tree Of Life platform (iTOL; http://itol.embl.de/index.shtml) after bootstrap analysis with 1000 replicates84.
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