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MKN45 total RNA (10 μg) was incubated for 15 min at 37 °C with or without 3 U/μg RNase R (Epicentre Technologies, Madison, WI, USA), and RNA was subsequently purified through phenol-chloroform extraction as described previously [18].
Copyright and License information: The Author(s) ©2020
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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