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Phospho‐Pbs2 band‐shift assay was conducted essentially as phospho‐Hog1 band‐shift assay except that Pbs2‐HA was first concentrated by immunoprecipitation from cell extracts before subjected to Phos‐tag SDS–PAGE.
Copyright and License information: ©2020 The Authors. Published under the terms of the CC BY 4.0 license
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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