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Sample preparation

SM Shu Meng
WX Wenchao Xia
MP Meng Pan
YJ Yangjie Jia
ZH Zhanlong He
WG Wei Ge
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Proteins were extracted from the right hippocampus of rhesus macaques using of the liquid nitrogen homogenization method [14]. Tissues were homogenized in ice-cold lysis buffer (8 M urea and 1× cocktail in PBS, pH 8.0) and then transferred to a 1.5-mL tube on ice for centrifugation at 12,000 rpm (15 min, 4 °C) to remove cellular debris. Subsequently, the supernatant was transferred to a fresh 1.5-mL tube. Protein concentrations of the supernatants were determined using the NanoDrop 2000 (Thermo Scientific, NJ, USA) according to the manufacturer’s instructions. Supernatants from each group were combined in equal amounts. Supernatants were immediately stored at − 80 °C.

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