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Protein expression analysis

SD Sergey A. Dyshlovoy
KO Katharina Otte
WA Winfried H. Alsdorf
JH Jessica Hauschild
TL Tobias Lange
SV Simone Venz
CB Christiane K. Bauer
RB Robert Bähring
KA Kerstin Amann
RM Ramin Mandanchi
US Udo Schumacher
JS Jennifer Schröder-Schwarz
TM Tatyana N. Makarieva
AG Alla G. Guzii
KT Kseniya M. Tabakmakher
SF Sergey N. Fedorov
LS Larisa K. Shubina
IK Igor E. Kasheverov
HE Heimo Ehmke
TS Thomas Steuber
VS Valentin A. Stonik
CB Carsten Bokemeyer
FH Friedemann Honecker
GA Gunhild von Amsberg
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Intracellular protein expression was analyzed using Western blotting. Cell treatment, protein extraction and Western blotting were performed as described previously [30]. Tumor samples were flash-frozen directly after tumor excisions and homogenized on ice prior to protein extraction. The antibodies used are listed in Supplementary information (Supplementary Table S1).

Copyright and License information:  ©2016 Dyshlovoy et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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