3.4. Preparation of Pigmented Isotonic Beverage Prototype (PIBP)

Five independent aliquots of colorless commercial isotonic beverages were selected: 0.53, 0.99, 1.55, 2.08 and 2.52 mg/g. The color difference among these prototypes of the colored beverages was determined. To perform the stability analysis with a commercial isotonic beverage (which had the addition of artificial colorants), the prototype with the least color difference was chosen.

An isotonic beverage pigmented with AOE at 1.55 mg/mL was prepared for the stability analysis, and the mixture was homogenized by stirring with an ULTRA-TURRAX at 35,000 rpm. This formulated beverage was called the pigmented isotonic beverage prototype (PIBP).