Female C57B6L/J mice aged 8 weeks were purchased from the Institute of Animal Research at Nanjing Medical University. The Ethics Committee of Nanjing Medical University (approval number: 20170480) approved this study. Mice were equally and randomly sorted into 4 groups: a normal group with no treatment (NG, n = 10), a POI group treated with CTX followed by the transplantation of PBS (POI, n = 10), an hAECs group treated with CTX following transplantation of hAECs (hAECs, n = 10), and a VC-hAECs group treated with CTX following transplantation of the VC-treated hAECs. CTX was administered by intraperitoneal injection at a concentration of 120 mg/kg.
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