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Equal amounts of extracted RNA from different seed maturation stages were pooled and used for cDNA library construction. To purify mRNA from 5 μg total RNA, the mRNA-Only Eukaryotic mRNA Isolation Kit (Epicentre) was used by applying exonuclease digestion followed by LiCl precipitation. One μg mRNA was used for the synthesis of the first-strand cDNA by the Mint-Universal cDNA Synthesis Kit (Evrogen). The Trimmer Kit (Evrogen) was used for normalization reaction using 800 ng amplified cDNA, which was re-amplified by 18 cycles.
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