2.5. Proteins involved in synthesis of secondary metabolites, vitamins and transporters

A unique pipeline was developed to identify the enzymes involved in biosynthesis of medicinally important secondary metabolites and vitamins. The protein queries were identified from PlantCyc database [14] for each enzyme. These queries were aligned using Clustal Omega [15] and the alignment was used to jump-start PSI-BLAST (E-value: 10⁻⁵, 2 iterations) [16] for identifying M. oleifera protein hits. Validation of hits was performed using phylogenetic analysis and functionally important residues mapping. The abundance of the transcripts, encoding these proteins was checked across different tissues.