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HeLa or Mettl3Mut/− cells were seeded on six-well plate one day before treatment. A total of 5 µg/mL actinomycin D (Act-D) was added in serum-free culture medium for the indicated times. Cells were washed by PBS and subjected to isolate total RNA by Trizol. RNA concentrations were quantified by a Qubit® RNA HS Assay Kit (ThermoFisher Scientific) and qRT-PCR performed, where 18S mRNA acted as the internal control.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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