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For knockdown of EGFR, Human Kinase EGFR (siRNA1) MISSION® siRNA (SIHK0657; Sigma-Aldrich) was used. Transfection of siRNA was performed using the Lipofectamine 2000 transfection reagent (Invitrogen) following the manufacturer protocol and assayed for silencing 24 and 48 hours after transfection.
Copyright and License information: ©2016 Fertig et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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