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2.8. Masson’s Trichrome Staining for Evaluation of Renal Interstitial Fibrosis
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Five-micrometer kidney slides were stained with Masson’s trichrome (Bioss Biological Technology Company, Beijing, China) according to the procedure outlined in the staining kit. After the staining, 20 fields were randomly selected on each slice to evaluate interstitial fibrosis (magnification: ×200). The results were calculated by Image Pro Plus 5.0 analysis software (Media Cybernetics, Silver Spring, USA) as the ratio of Masson positive stained area to the total field area.
Copyright and License information: ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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