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2.5. Blood Glucose and Serum Insulin Measurement
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At the end of the experiment, the rats were sacrificed with an overdose of isoflurane after anesthesia. Blood glucose was measured from the tail vein using a glucometer (Roche Diagnostics, Indianapolis, IN, USA). Blood was taken from the heart and centrifuged for 10 min at 4 °C to extract serum; an ELISA kit was used to detect serum insulin.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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