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Determination of CSF glutamine levels.
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Glutamine levels in human CSF samples were determined using the Glutamine Detection Assay Kit (ab197011, Abcam) following the manufacturer’s instructions. The assay is based on the hydrolysis of glutamine to glutamate, which produces a stable signal that is directly proportional to the amount of glutamine in the sample. Experimental values were interpolated in Excel within a standard curve of 0, 2, 4, 6, 8, and 10 nmol/well.
Copyright and License information: ©2020 American Society for Clinical Investigation
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