Measurement of Antioxidant Enzyme Activities

The activities SOD, CAT, GR, GPx, and GST were detected in this study. SOD activity was detected according to the instructions in the enzyme activity assay kit (WST-1). Lymphocytes were obtained and homogenized by centrifugation at 640 × g for 15 min at 4°C in cold PBS to obtain the supernatant. The mixture was incubated at 37°C for 20 min. Finally, using a multimode microplate reader (Infinite M1000), the absorbance was measured at 450 nm. The sample activity was expressed in unit/mg protein. The activities of other enzymes, namely, CAT, GR, GPx, and GST, were detected according to the instructions indicated in the corresponding assay kits.