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Measurements of the CEMP1-p4 solution were acquired with a Zetasizer Nano S (Malvern Instruments, Ltd., Clevedon Organs, UK) molecular sizing instrument [75]. The temperature was held at 37 (0.1 °C) with a Peltier unit. Data analyses were performed using the software Zetasizer Nano Family age (V.7.10), (Malvern Instruments, Ltd., Clevedon Organs, UK).

In silico analyses—the isoelectric point (pI), molecular weight, hydrophobic character and GRAVY of the CEMP1-p4 peptide were determined by using the ExPASy tool ProtParam. (https://web.expasy.org/protparam/).

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