3.3. GC/MS Analysis of Volatile Compounds

One µL of the extracts was injected into the GC/MS-TOF (gas chromatography time of flight) (Pegasus III, Leco, St. Joseph, MI) using the auto sampler. Compounds’ separation was carried out on a capillary column (DB-5MS UI, 10 m long, 0.180 mm id and 0.18 µm film thickness (Hewlett Packard, Palo Alto, CA)) for three minutes at 40 °C and then raised at 30 °C min⁻¹ to 250 °C and hold for two minutes. The used carrier gas was helium and its flow rate was set to 3 mL min⁻¹ (for two minutes) and 1.5 mL min⁻¹ afterwards. The mass spectrometry setting was prepared for generating a mass spectrum at 70 eV with a 90 s solvent delay at 1597 eV at 20 scans/second. The selected mass range was 50 to 350 atomic mass units (AMU).