4.4. In vitro Antifungal Assay

All the isolated actinomycetes were tested in an antagonistic assay conducted using the dual-culture technique. A 5 mm agar plug of Pyricularia sp. was placed on a side of the plate and another 5 mm agar plug with an actinomycete was placed on the opposite side [34]. Plates were incubated at 28 °C for 5 days and the antagonistic activity was scored according to the scale developed by Alfredo and Aleli (2011) [35]. The diameter of the mycelium growing out from the plug was measured and reported as the percentage inhibition of radial growth (PIRG) using the following formula [36]

where R 1= Radial growth of Pyricularia sp. (mm) in the control plates

Three biological replicates were performed and an average was taken.