The mouse model of nonalcoholic fatty liver disease (NAFLD) induced by a high-fat diet

Male Institute of Cancer Research (ICR) mice (age, 6–8 weeks) were purchased from Beijing Wei Tong Li Hua Laboratory Animal Technology Co., Ltd. and housed in standard conditions at 24–26°C, with a 12-hour light and dark cycle. The animals had free access to food and water. The animal experiments were conducted according to a protocol approved by the Animal Care and Ethics Committee of the First Central Hospital of Tianjin.

The high-fat diet and the regular mouse diet were obtained from Meidisen Biomedical Company (Jiangsu, China). The caloric content of the high-fat diet was composed of 20% carbohydrate, 20% protein, and 60% fat. The caloric content of the regular mouse diet was composed of 70% carbohydrate, 20% protein, and 10% fat.