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Animal dosing and sample collection for pharmacokinetic studies
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Three mice were administered a single IP dose of 11a-1 at 10 mg/kg. At different time points (1 hour, 3 hours, 6 hours, 9 hours and 24 hours), blood samples (50 μl) from each mouse were collected and mixed with EDTA (50 mM, 50 μl). The samples were then mixed with 200 μl acetonitrile and centrifuged at 21,100 g for 5 minutes. The supernatants were collected and subjected to liquid chromatography/mass spectrometry analysis.
Copyright and License information: ©2016 Zhang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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