2.5. Determination of hepatic GSH, SOD activities and MDA content

Livers of treated animals were surgically removed and perfused in ice-cold normal saline (0.9% NaCl). The fresh tissue was used to prepare 10% homogenate in 0.1 M Tris HCl buffer (pH 7.4) and was used for the determination of protein content (Lowry et al., 1951). Concentration of MDA in tissue was determined according to the method previously described by Ohkawa et al. (1979). Glutathion concentration was measured according to previously described method by Teitz (1967), and the activities of SOD according to Misra and Fridovich (1972). Reaction products were measured spectrophotometrically.