Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
A 10 mg/ml solution of 4-hydroxy-tamoxifen was prepared in a mixture of autoclaved sunflower oil (9 part) and ethanol (1 part) with end-to-end rotation for 1 h at room temperature and stored at 4 °C in a light-proof container. Six-week old mice were injected intraperitoneally with 2 mg (200 uL) tamoxifen for 3 subsequent days. Control mice received equal volume of the oil-ethanol vehicle. Mice were processed for immunostaining after 2 weeks of injections.
Copyright and License information: The Author(s) ©2020
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
