4.4. Experimental Approach for Solubility Determination

The solubility of GLZ in all studied solvents was determined using an experimental approach such as the “isothermal saturation shake flask method” at T = 298.2–318.2 K and p = 0.1 MPa.⁴⁶ The operational conditions and experimental procedures were same as mentioned in our previous work.^40,45 Therefore, the experimental details are not included in the present study. Experiments were carried out in triplicate manner (n = 3). The content of GLZ in the solubility samples of 12 different neat solvents was analyzed by the UPLC-UV technique described above at λ_max = 230 nm. The content of GLZ (μg g^–1) was estimated using a calibration curve of GLZ. The x_e values of GLZ were calculated by applying eq 1(26,27)

where m₁ = mass of GLZ; m₂ = mass of neat solvent; M₁ = molar mass of GLZ; and M₂ = molar mass of neat solvent.