Determination of the serum malondialdehyde (MDA) level

GW Geng-Chin Wu
CP Chung-Kan Peng
WL Wen-I Liao
HP Hsin-Ping Pao
KH Kun-Lun Huang
SC Shi-Jye Chu
ask Ask a question
Favorite

The extent of serum lipid peroxidation was determined by measuring the concentration of thiobarbituric acid-reactive substances (TBARS) using a TBARS ELISA kit (Cayman Chemical Co., Ann Arbor, MI, USA). Standards or samples were boiled for 1 hour at 100 °C and centrifuged at 16,000×g for 10 min. The absorbance was recorded at 532 nm.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A