BV2 Cell Culture

The murine microglia cell line BV2 was preserved in DMEM/F12 (Thermo Fisher Scientific, Germany) supplemented with 10% heat-inactivated FCS and 1% penicillin/streptomycin (PAA, Cölbe, Germany), and the cells were incubated at 37°C in a 5%-CO₂-containing 95% humidified air atmosphere. Before the treatments with 5 ng/ml TGFβ1 (Peprotech, Hamburg, Germany) for ChIP experiments, 1 × 10⁶ cells/10 cm dish were washed twice with PBS and kept under serum-free conditions for at least 2 h.