Intravitreal injection

In this experiment, 54 mice were randomly divided into three groups (n=18/group): Control, vehicle and PFD. The control group and the remaining 9 mice (normal group) received no treatment. The injury induced by CNV and the potential toxicity of PFD in the control, vehicle and PFD groups were compared to the normal group (4 mice were used in choroidal flat mount experiment and 5 mice were used in the histopathological examination, respectively). As described in a previous experiment (25), an intravitreal injection of 1 µl 0.5% PFD (Beijing Kangdini Pharmaceutical Co., Ltd.), or vehicle (0.01 M PBS solution: Sodium chloride, 137 mM; disodium phosphate dodecahydrate, 9 mM; and sodium dihydrogen phosphate dehydrate, 2.9 mM) was administered on day 0 to the PFD and vehicle group, respectively. Mice were decapitated at day 7 and 28 following anesthesia (5% isoflurane).