Animal model of endotoxemia.

Mice were injected i.p. with 15 mg/kg BW of LPS (Escherichia coli serotype O55:B5; MilliporeSigma). Mice were allocated to the treatment or vehicle group. Treatment mice received an i.p. injection of 10 mg/kg BW M3 at the time of LPS injection. The vehicle group received an equivalent volume of PBS. After 90 minutes, mice were sacrificed, and serum was collected for analysis. To study survival rates, mice were treated with 15 mg/kg BW LPS with or without 10 mg/kg BW M3 and were evaluated twice daily for 7 days.