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Serum IgG and IgM

WL Wai Man Lio
BC Bojan Cercek
JY Juliana Yano
WY Wei Yang
JG Jonathan Ghermezi
XZ Xiaoning Zhao
JZ Jianchang Zhou
BZ Bo Zhou
MF Michael R. Freeman
KC Kuang-Yuh Chyu
PS Prediman K. Shah
PD Paul C. Dimayuga
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Mouse serum was diluted 1:50 and 1:100 with 1X PBS for the detection of IgG and IgM against peptide, respectively, by ELISA using standard protocol on Maxisorp 96 well-plates coated with peptide (LifeTein) at 20 μg/ml in Na2CO3-NaHCO3 buffer, pH 9.6. Two percent goat serum in 1X PBS was used for blocking and dilution of biotin-conjugated anti-mouse IgG (Thermo Scientific) or IgM (SouthernBiotech) antibodies in 1:5000 and 1:10000 weight to volume dilutions, respectively. Detection was with avidin-HRP (eBiosciences) and ABTS substrate (SouthernBiotech) analyzed at 405 nm.

Copyright and License information:  ©2020 Lio, Cercek, Yano, Yang, Ghermezi, Zhao, Zhou, Zhou, Freeman, Chyu, Shah and Dimayuga.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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