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Lipid content

KT Kiruthika Thangavelu
PS Pugalendhi Sundararaju
NS Naganandhini Srinivasan
IM Iniyakumar Muniraj
SU Sivakumar Uthandi
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The yeast biomass (100 mg) was homogenized with 20 volumes of chloroform:methanol mixture (2:1; v:v) and agitated overnight in an orbital shaker at room temperature. The lipid phase was separated by adding 0.2 volume of distilled water and centrifuged at 3000 rpm for 10 min. The upper phase was siphoned out, and the lower chloroform phase containing lipids was evaporated under vacuum. The extracted lipids were measured gravimetrically [48].

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