In each patient, the preoperative ECD of the donor graft was obtained from the donor information provided by SightLife Eye Bank, and at every 6 months postoperatively, ECD was measured by use of a non-contact specular microscope (EM-2000 or EM-3000; TOMEY, Nagoya, Japan). Corneal graft failure was defined as irreversible corneal oedema. Cases with graft failure, and cases with a clear graft in which clear specular microscopy images could not be obtained, were excluded from the postoperative ECD analysis. The donor graft survival rate was recorded every 6 months postoperatively, and was defined as the percentage of clear grafts.
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