5.1.7. Social Interaction Test

A social interaction test was carried out to assess the zebrafishes’ ability to interact with their conspecific. Similar to the predator avoidance test, a glass separator was used in the same size tank as described in the previous publication [59]. After acclimation, the tested fish were introduced in one side of the tank with a conspecific in another side. After video recording, several important endpoints (interaction time percentage, longest duration in the separator side, average speed, and average distance to the separator) were calculated.