IFN-γ ELISA

JW Jason B. Williams
SL Shuyin Li
EH Emily F. Higgs
AC Alexandra Cabanov
XW Xiaozhong Wang
HH Haochu Huang
TG Thomas F. Gajewski
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For IFN-γ ELISA, tumors were isolated, separated into two sections and weighed. One section was dissociated using frosted slides in 500 μl cold PBS and pelleted at 21,130 × g for 10 min at 4 °C. Fifty microliter of supernatant was used for IFN-γ ELISA (R&D) following manufacturer’s protocol.

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