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The Luciferase Assay System was used to perform transcriptional activity assays. Cells were co-transfected with FOPflash or TOPflash with pRL purchased from Millipore Corporation. After transfection for 24 h, the cells were harvested and subjected to luciferase activity measurement on a BioTek luminometer using the Dual-Luciferase Reporter Assay System purchased from Promega Corporation. The ratio of firefly luciferase activity to Renilla luciferase activity was calculated to evaluate the transcriptional activity of each sample.
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