2.4. Formalin-ether concentration technique (FECT)

The portion of intestinal content was collected using an applicator stick and transferred into a beaker. Eight milliliters (8 mL) of normal saline was added and stirred. The mixture was then filtered using funnel and gauze into a 15 mL centrifuge tube. The filtrate was obtained for further studies while the lumpy residues were discarded. The filtrate was later centrifuged at 1500 rpm for 5 min following which, the supernatant was discarded. Seven milliliters (7 mL) of 10% formalin solution was added into the tube followed by 3 mL ethyl-acetate. The mixture was thoroughly mixed and centrifuged for 10 min at 1500 rpm. Three layers; ethyl-acetate, formalin, and sediment were formed. The two layers; formalin and ethyl-acetate were carefully discarded while the sediment was retained. This was gently mixed with a rubber pipette and a drop was placed onto a clean, grease-free glass slide, stained with iodine, and covered with a coverslip (Anne and Conboy, 2012).