24, 48, and 72 h after injection, mice were anaesthetized with ketamine and dopamine anesthetic mix (as recommended by the CULATR, 25:1 ratio), and 100 μL of luciferin substrate (30 mg/mL, Gold Biotechnology) was injected intraperitoneally into the mice. Within 5 min of substrate delivery, mice were viewed under the in vivo imager (IVIS SPECTRUM) and luciferase signals were detected.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.