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S-OPA1 and Δ196–252 was diluted to 0.3 mg/ml in 50 mM HEPES (pH 7.4), 300 mM NaCl and 1 mM DTT. The amine-reactive crosslinker bis(sulfosuccinimidyl) suberate (BS3; Thermo Fisher Scientific) was added to a final concentration of 50 μM. After a 15 min incubation at room temperature, the crosslinking reaction was quenched with 50 mM Tris (pH 8.0). Crosslinked products were analyzed using a 3–8% Tris–acetate PAGE.
Copyright and License information: ©2020, Zhang et al
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