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To generate Pin1-depleted cells, cells were infected with retrovirus vector carrying Pin1-specific shRNA (Ryo et al., 2005). For the production of retroviruses, Plat-E cells (Morita et al., 2000) were transduced with pSUPER.retro vector and pVSV-G with Effectene reagent (Qiagen). After 48 h, cell supernatants were filtrated with a 0.45-μm filter and added with 10 μg/ml Polybrene. Target cells were then selected with 1 μg/ml puromycin (InvivoGen).
Copyright and License information: ©2020 Nishi, Miyakawa, Matsunaga, Khatun, Yamaoka, Watashi, Sugiyama, Kimura, Wakita and Ryo.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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