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Construct Generation and Plant Transformation

CN Candida Nibau
MG Marçal Gallemí
DD Despoina Dadarou
JD John H. Doonan
NC Nicola Cavallari
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For transient expression in Nicotiana benthamiana leaves, the CDKG2-GFP (Cavallari et al., 2018) and RSp34-RFP (Lorković et al., 2004) cassettes were cloned into the pEAQ-HT-DEST2 vector (Sainsbury et al., 2009) and transformed into Agrobacterium tumefaciens strain LBA4404. Leaf infiltration was performed as described (Sainsbury et al., 2009). After 5 days, leaves were harvested for confocal imaging using a Leica TCS SP5 II confocal laser scanning microscope (CLSM) controlled by Leica LAS-AF software.

Copyright and License information:  ©2020 Nibau, Gallemí, Dadarou, Doonan and Cavallari
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