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ABTS radical scavenging activity

GM Garland K. More
RM Raymond T. Makola
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The determination of free radical scavenging activity of plant extracts and ascorbic acid was done by ABTS radical cation decolorization assay. ABTS cation radical production was accomplished by combining 10 mg of ABTS and 2 mg potassium persulfate in water. The solution was kept in the dark at room temperature for approximately, 12–16 h before use. ABTS solution (1 mL) was then diluted with 60 mL of methanol. The experiment was conducted following the method described in the DPPH Scavenging Activity Section. Measurements were then gradually carried out in triplicates. The percentage of inhibition of absorbance at 734 nm was calculated using the formula stated by Prior et al.36.

where control A0. and sample A1. are absorbances of the ABTS without the extract and DPPH with the extract, respectively.

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