2.7. C. elegans Lifespan Assay

To obtained a synchronized worm population, N2 adults were placed to lay embryos for 8 h on NGM plates containing a lawn of US-7, IT-3 samples, or E. coli OP50, and then sacrificed. All lifespan assays started when the progeny became fertile (t0). Animals were transferred to new plates seeded with fresh lawns and monitored daily. They were scored as dead when they no longer responded to gentle touch with a platinum wire. At least 60 nematodes per condition were used in each experiment.