VEGFR2 kinase activity was measured in vitro using the VEGFR2 (KDR) Kinase Assay kit (BPS Bioscience). Fasentin was tested at 100 µM according to the manufacturer’s instructions, determining the percentage of remaining kinase activity after 45 min incubation at 30 °C. 1 µM sunitinib was used as positive control of inhibition24,25. Data were collected using a GloMax-96 microplate luminometer (Promega).
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