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Characterization of pyocyanin by Liquid Chromatography–Mass Spectrometry (LCMS)
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An aliquot of standard and extracted pyocyanin was used for the LCMS analysis. Ten µl of analyte was subjected to LC (Aquity, Waters) equipped with BEH C18 of 1.7 µm of a 1.0 × 50 mm column and followed by mass spectrometry, the mass range being ionized by the Synapt G2 Waters. An analyte prepared with 0.1% formic acid in water in combination with acetonitrile was subjected to LC at controlled room temperature and column temperature of 50 °C in gradient mode for 8 mins reverse phase.
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