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The proliferation of BGC-823 and MKN45 cells was evaluated using cell counting kit-8 (CCK-8) assay (Dojindo, Kumamoto, Japan). In brief, cells were seeded in 96-well plates at 2000 cells/well and cultured for 24, 48, 72, 96, and 120 h. Then, the cells were incubated with 10 μL of CCK-8 solution at 37°C, and the absorbance at 450 nm was calculated.
Copyright and License information: ©2020 Wu et al.
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