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Cell Migration Assay (Wound Healing Assay)
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Cell migration capacity was measured by wound-healing assay. Cells were grown in six-well plates until reaching 90% confluency. A sterile 200 μL pipette tip was used to scratch a vertical line on the plate. Cells were washed with PBS twice. Then, cells were treated with serum-free 1640 containing indicated ingredients for 48 h. Images were obtained in triplicate for each condition using an inverted microscope at 0 h (baseline) and 48 h (end-point). The wound-healing area was calculated by Image J software.
Copyright and License information: ©2020 Lu et al.
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