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Analysis of cholesteryl glucosides

HJ Hau-Ming Jan
YC Yi-Chi Chen
TY Tsai-Chen Yang
LO Lih-Lih Ong
CC Chia-Chen Chang
SM Sasikala Muthusamy
AA Andualem Bahiru Abera
MW Ming-Shiang Wu
JG Jacquelyn Gervay-Hague
KM Kwok-Kong Tony Mong
CL Chun-Hung Lin
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These experiments were performed as previously described14. For ultrahigh-performance liquid chromatography (UPLC, Waters)-MS (Orbitrap Elite, Thermo), a CSM C-18 column was used with a gradient from 30% acetonitrile (ACN) with 20 mM ammonium acetate to a mixture of 90% isopropyl alcohol and 10% ACN with 20 mM ammonium acetate. The mass spectrometer was operated in positive electrospray ion mode set to one full FT-MS scan (m/z 200–1600; 15,000 resolution) and switched to different FT-MS product ion scans (15,000 resolution) for the different precursors of cholesteryl glucoside derivatives. Both collision-induced dissociation and higher energy collisional dissociation were utilized to perform the fragmentation. For sensitive quantitation, multiple reaction monitoring was performed, which detected the fragment of MAN at 366.16 m/z.

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