2.2. Apple Samples

Around two kg of each variety of apple were harvested from orchards in Croatia (an orchard in the town Donji Mihaljevci (45°22’47.6”N 17°40’34.3”E) owned by Mirko Veić, and orchards in the town Gornji Tkalec (45°58’24.0”N 16°27’12.0”E) and the village Rude (45°46’35.6”N 15°40’35.8”E)) in the following varieties: ‘Crvenka’, ‘Crveni Boskop’, ‘Pisanika’, ‘Lještarka’, ‘Božićnica’, ‘Božićnica 2’, ‘Cox’s Orange Pippin’, ‘Ivanlija’, ‘Boskop’, ‘Bobovac’, ‘Slavonska srčika’, ‘Kolerova srčika’, ‘Batulenka’, ‘Gravenstein’, ‘Kandil Sinap’, ‘Citronka’, ‘Zimnjara’, ‘Zlatica’, ‘Mašanka’, ‘Kanada’, ‘Gloria Mundi’, ‘Zelenika’, ‘Krastavka’, ‘Adamova zvijezda’, and ‘Wild apple’. The variety Božićnica was harvested from two orchards (Donji Mihaljevci and Gornji Tkalec), so the names used here are ‘Božićnica’ and ‘Božićnica 2’, respectively. The commercial variety ‘Idared’ was harvested in the orchard in the village of Rude (Samobor). This variety was chosen because it is one of the most often grown and popular commercial varieties in Croatia and in this study it served as a control. Apples of each variety were divided into two groups. The group of apples for quality determination consisted of approximately one kg of each variety. It was transferred to the laboratory of the Faculty of Agriculture, University of Zagreb, and kept in a refrigerator before internal and external quality traits were determined. The other group of approximately one kg of each variety was transferred to the laboratories of the Faculty of Food Technology, University of Osijek, where they were kept in a refrigerator for no more than 5 days before extracts for the determination of polyphenolic compounds were made. Polyphenol contents of the varieties ‘Bobovac’, ‘Batulenka’, ‘Mašanka’, and ‘Krastavka’ were never studied before to the best of our knowledge. The quality traits and polyphenolic compounds of all varieties were never studied before in so much detail.