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In vivo Bioluminescence Imaging of Mice
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Mice were anesthetized with 2% isoflurane and placed in a light-tight chamber. A photographic (gray-scale) reference image was obtained at 5 min after D-luciferin (Sigma) injection (150 mg/kg i.p.); bioluminescent images were collected immediately thereafter. Bioluminescence of the mice was detected via the IVIS Imaging System 200 Series. The region of interest from displayed images was designated and quantified as total flux (photons/sec) using Living Image 2.50 software (Xenogen).
Copyright and License information: ©2020 Cheng, Lee, Oh, Furtmüller, Patel, Brandacher and Powell.
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