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Site-directed mutagenesis

XX Xiaolei Xie
GL Guillermina Lozano
ZS Zahid H. Siddik
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The mutations R172V and L344P were introduced into p53 by using QuikChange Lightning Multi Site-Directed Mutagenesis Kit, following the manufacturer’s instruction. The primers for the mutagenesis are as follows:

5′-AGCACATGACGGAGTTTGTGAGGCGCTGC-3′ and 5′-GCAGCGCCTCACAAACTCCGTCATGTGCT-3′ for R172V; 5′-GATGTTCCGAGAGCCGAATGAGGCCTTGGAACTCAAGG-3′ and 5′-CCTTGAGTTCCAAGGCCTCATTCGGCTCTCGGAACATC-3′ for L344P.

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