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In vitro kinome profiling measuring drug binding
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The DiscoveRx KinomeScan platform (https://www.discoverx.com), utilizing an in vitro active site-directed competition binding assay, was used to quantitatively measure interactions between the four clinical PARP inhibitors and their largest available kinase panel. At the time when the assays were performed (date: 29/11/2016), 468 in vitro binding assays were available, corresponding to 392 unique human kinases (76% of the human kinome)39 (Supplementary Table 5).
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